Nod2 Deficiency in mice is Associated with Microbiota Variation Favouring the Expansion of mucosal CD4+ LAP+ Regulatory Cells

Nucleotide-binding Oligomerization Domain-2 (NOD2) mutations are associated with an increased risk to develop Crohn's Disease. In previous studies, we have shown that Nod2-/- mice manifest increased proportion of Lamina Propria (LP) CD4+ LAP+ Foxp3- regulatory cells, when compared with Nod2+/+ mice, while CD4+ Foxp3 + regulatory cells were not affected. Here, we investigated the Nod2 gut microbiota, by 16S rRNA pyrosequencing, at steady state and after TNBS-colitis induction in mice reared separately or in cohousing, correlating the microbial profiles with LP regulatory T cells proportion and tissue cytokines content. We found that enrichment of Rikenella and Alistipes (Rikenellaceae) in Nod2-/- mice at 8 weeks of age reared separately was associated with increased proportion of CD4+ LAP+ Foxp3- cells and less severe TNBS-colitis. In co-housed mice the acquisition of Rickenellaceae by Nod2+/+ mice was associated with increased CD4+ LAP+ Foxp3- proportion and less severe colitis. Severe colitis was associated with enrichment of gram-negative pathobionts (Escherichia and Enterococcus), while less severe colitis with protective bacteria (Barnesiella, Odoribacter and Clostridium IV). Environmental factors acting on genetic background with different outcomes according to their impact on microbiota, predispose in different ways to inflammation. These results open a new scenario for therapeutic attempt to re-establish eubiosis in Inflammatory Bowel Disease patients with NOD2 polymorphisms

Misure di resistività mediante OhmMapper finalizzate allo studio del sito di Torraccia di Chiusi

The paper describes the findings of a geo-electric study conducted at the archaeological site of Torraccia di Chiusi (Siena Province) with the use of soil resistivity modelling. In collaboration with the Département d’Archéologie et Histoire de l’Art (Université catholique de Louvain, Belgium) and the Centro di GeoTecnologie (Università degli Studi di Siena), a new, non-invasive instrument, named the OhmMapper, was used for the first time in an archaeological context to provide geophysical sections of the terrain. The aim of the study was to evaluate the nature and distribution of the buried anthropogenic structures and to properly locate them on the map without the necessity of excavating. The site, located in the valley of the Foci river, has been described as a long lasting settlement (III to VII centuries A.D., although ceramics dating to the II century B.C. have been uncovered). Belgian and Italian archaeologists, who have been digging at the site for the last two years, have also discovered that it had undergone multiple additions and modifications throughout the years (initially a villa, and later perhaps a mansio). The OhmMapper is a capacitively coupled resistivity meter that measures the electrical properties of rocks and soil without ground stakes used in traditional resistivity surveys. A simple coaxial-cable array with transmitter and receiver sections is pulled along the ground either by a single person or attached to an all-terrain vehicle. The dipole-dipole configuration enables the instrument to immediately pick up the horizontal variations in resistivity, thereby enabling the user to identify structures such as walls or cavities. With the OhmMapper, nine sections were made around the perimeter of the area already excavated to collect data on the presence, depth, and dimensions of anthropogenic structures. The results from the sections were refined and placed on a map using an ArcGIS platform. The interpretation of the nine sections resulted in a new hypothesis on the buried anthropogenic structures and a new model of the natural landscape that existed at the site prior to its construction

Antifungals, arthropods and antifungal resistance prevention: lessons from ecological interactions

Arthropods can produce a wide range of antifungal compounds, including specialist proteins, cuticular products, venoms and haemolymphs. In spite of this, many arthropod taxa, particularly eusocial insects, make use of additional antifungal compounds derived from their mutualistic association with microbes. Because multiple taxa have evolved such mutualisms, it must be assumed that, under certain ecological circumstances, natural selection has favoured them over those relying upon endogenous antifungal compound production. Further, such associations have been shown to persist versus specific pathogenic fungal antagonists for more than 50 million years, suggesting that compounds employed have retained efficacy in spite of the pathogens' capacity to develop resistance. We provide a brief overview of antifungal compounds in the arthropods' armoury, proposing a conceptual model to suggest why their use remains so successful. Fundamental concepts embedded within such a model may suggest strategies by which to reduce the rise of antifungal resistance within the clinical milieu

biologically costly altruism depends on emotional closeness among step but not half or full sibling

We studied altruistic behaviors of varying biological cost (high, medium, and low) among siblings of varying genetic relatedness (full, half, and step). In agreement with inclusive fitness theory, the relative importance of either reliable (such as co-residence) or heuristic (such as emotional closeness) kinship cues depended crucially on the costs of help. When help did not endanger the altruist's life, thus making reciprocation possible, emotional closeness was the strongest predictor of altruism; perceived physical and psychological similarity to the sibling amplified altruistic behavior via their association with emotional closeness. When help endangered the altruist's life, thus making reciprocation unlikely, the strongest predictor of altruism was the ancestrally valid kinship cue of co-residence duration. Emotional closeness predicted costly altruism only for step siblings; its effects were non-significant when siblings were genetically related. Our findings support the idea that emotional closeness promotes costly altruistic behavior by serving as a surrogate kinship cue when more reliable cues are missing

cis-Regulatory sequences driving the expression of the Hbox12 homeobox-containing gene in the presumptive aboral ectoderm territory of the Paracentrotus lividus sea urchin embryo.

Embryonic development is coordinated by networks of evolutionary conserved regulatory genes encoding transcription factors and components of cell signalling pathways. In the sea urchin embryo, a number of genes encoding transcription factors display territorial restricted expression. Among these, the zygotic Hbox12 homeobox gene is transiently transcribed in a limited number of cells of the animal-lateral half of the early Paracentrotus lividus embryo, whose descendants will constitute part of the ectoderm territory. To obtain insights on the regulation of Hbox12 expression, we have explored the cis-regulatory apparatus of the gene. In this paper, we show that the intergenic region of the tandem Hbox12 repeats drives GFP expression in the presumptive aboral ectoderm and that a 234 bp fragment, defined aboral ectoderm (AE) module, accounts for the restricted expression of the transgene. Within this module, a consensus sequence for a Sox factor and the binding of the Otx activator are both required for correct Hbox12 gene expression. Spatial restriction to the aboral ectoderm is achieved by a combination of different repressive sequence elements. Negative sequence elements necessary for repression in the endomesoderm map within the most upstream 60 bp region and nearby the Sox binding site. Strikingly, a Myb-like consensus is necessary for repression in the oral ectoderm, while down-regulation at the gastrula stage depends on a GA-rich region. These results suggest a role for Hbox12 in aboral ectoderm specification and represent our first attempt in the identification of the gene regulatory circuits involved in this process

Adaptive Dispersion Compensation for Remote Fiber Delivery of NIR Femtosecond Pulses

We report on remote delivery of 25 pJ broadband near-infrared femtosecond light pulses from a Ti:sapphire laser through 150 meters of single-mode optical fiber. Pulse distortion due to dispersion is overcome with pre-compensation using adaptive pulse shaping techniques, while nonlinearities are mitigated using an SF10 rod for the final stage of pulse compression. Near transform limited pulse duration of 130 fs is measured after the final compression.Comment: 3 pages, 4 figure

Avaliação da seletividade inicial de herbicidas para o tomateiro por meio da análise de fluorescência da clorofila.

Objetivou-se com este trabalho analisar a fluorescência da clorofila por meio da avaliação da taxa de transporte de eletrons em tomateiro como forma de avaliar a seletividade inicial de hervicidas aplicados em pré-transplate

Yeast as a model to investigate the mitochondrial role in adaptation to dietary fat and calorie surplus

Several research strategies are focused towards understanding the genetic basis and molecular mechanisms that regulate uptake, synthesis, deposition, and mobilization of lipids, in the context of energy homeostasis. Because of the complexity of the problem, major input comes from the use of model systems. The aim of this work was to test the feasibility of using yeast as a model organism for studies related to dietary challenges due to high fat diet and investigate the correlation between FA metabolism and oxidative metabolism. In particular, we ask to what extent the utilization of oleic acid is dependent on mitochondrial function. We studied growth on oleic acid as a sole carbon source, and oleate stress (growth in 2 and 5% oleate) in both laboratory (BY4741 wild-type and Δsco1, Δsco2, Δtgl3, Δtgl4 mutants) and natural strains, comparing the growth phenotypes with the respiratory behaviour for each strain. We confirmed that respiratory competence is fundamental for growth on oleic acid, since the respiratory deficient mutant Δsco1 was unable to grow on oleic acid. In order to understand if the ability to use oleate as carbon source and adapt to high oleate concentrations is a general trait for the Saccharomyces cerevisiae genus, we also studied some natural strains, both diploid and haploid, identifying two meiotic derivatives of SGU90 as unable to grow in oleic acid as a sole carbon source. We investigate some aspects of mitochondrial metabolism in order to gain insights on this new finding